BIOL 6961Genomics and ProteomicsFall 2001


 
Office: Science Center 3C44
Office: Science Center 3C13 
Phone: 276-8442
Phone: 276-6492
office hours by arrangement
office hours by arrangement

Date and Time: Monday afternoons from 1-4 (immediately following the Biology Department seminar)

Location: Science Center 2C25

Text: Special Human Genome Issue (15 February 2001)

Science (http://www.sciencemag.org/feature/data/genomes/landmark.shl)and

Nature (http://www.nature.com/genomics/human/)plus additional selected articles

Proteomics.SR Pennington and MJ Dunn, ed.BIOS Scientific Publishing LTD.2001.

Class Format: Lecture to providebackground information plus discussion of topics based on assigned reading.All students are expected to read the selected references before class.

Assessment and Grading

Presentation.A group of students will lead the class discussion during selected weeks of the semester.Each student will be responsible for leading discussion twice during the semester – once during the genomics section and once during the proteomics section. Dates will be chosen by random allotment.

Summary and Analysis of discussion.Each week another group of students will be responsible for providing a written summary of the class discussion.Each student will be responsible for two written summaries during the semester – once during the genomics section and once during the proteomics section.Summaries are to be written individually and will be posted in webct.

Grade will be based on:

presentation

participation in class discussions

summary and analysis

Academic Integrity:Student-teacher relationships are built on trust.For example, students must trust that teachers have made appropriate decisions about the structure and content of the courses they teach, and teachers must trust that the work that students do is their own.Acts which violate this trust undermine the educational process.The Rensselaer Handbook defines various forms of academic dishonesty and procedures for responding to them.All forms are violations of the trust between students and teachers.Students should familiarize themselves with this portion of the Handbook and should note that the penalties for plagiarism and other forms of cheating are quite harsh.


 
Schedule 
Part 1 – Genomics: Dr. Donna E.Crone
Aug 27
Introduction to the Human Genome Project
Sept 3-
No Class Labor Day
Sept 10
Physical Mapping and Sequencing of the genome
Sept 17 
Sequence Analysis and Annotation
Sept 24 
Sequence Analysis:comparative homologies, Evolutionary changes and Single Nucleotide Polymorphisms
Oct 1
Expression: analysis of expressed genes
Oct 9 (Tues= Mon)
Epigenetics: control of gene expression and how itsaffects proteins that are present in cell at any one time

Oct 15
Post Genomics.. 
Part 2 – Proteomics: Dr. Jane F. Koretz
Oct 22
Dr.Donita Garland, Chief, Protein Biochemistry Section

Laboratory of Molecular and Developmental Biology

National Eye Institute

topic:What is PROTEOMICS?

Overview:systems, time course, info, databases, post-translational modification, etc.

Oct 29
2-Dimensional gel electrophoresis

-Methods (including IPGs), 

-Resolution

-Reproducibility and image analysis

-Future developments

Nov 5
Mass spectroscopy

-Methods/strategies

-Protein identification

-De novo protein characterization

-Isotope labeling 

-N- and C-terminal tags

Nov 12
Other methods I

-Co- and post-translational modification 

-Automation 

-Creating and using the databases

Nov 19 
NO CLASS
Nov 26
Other methods II

-Microarrays

-Phage anitbodies

-Integrating proteomics and bioinformatics

Dec 3(last day of classes 12/7)
Biological and biomedical applications

-Drug discovery

-Diagnosis and treatment

-Etc.

last updated 8/25/01

Donna E. Crone