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1TRO: E. Coli Trp Repressor Protein Complex
In the Chime Exercise to the right, identify the following:
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the Helix-Turn-Helix motifs and the two recognition helices
in the major grooves
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The 18 bp palindromic sequence that is recognized by these
recognition helices.
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the Trp molecules on the protein
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Can you identify any direct contacts between the side
chains and the bases in the major groove that might account for
recognition specificity?
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Identify some of the H-bonding contacts between the side
chains and the non-esterified DNA backbone phosphate oxygens.
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