CHIME EXERCISE
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1DUD: Deoxyuridine-5'-Nucleotide Hydrolase (D-UTPase)
The structure to the right is dUTPase in a complex with a bound substrate analog, Deoxyuridine-5'-Diphosphate (dUDP).
(1) Find
the substrate in its binding site (2) Find
C5 on the Uracil group. Is there enough room to
attach a methyl group to C5? (3) Locate
the ribose 2’ C. What protein group sterically
prevents an –OH group from being attached to the 2’ C atom? (4) Find the H-bond donors and acceptors (to the uracil base) from the protein. What would be the effect on the H-bonding if the base was changed to cytosine? |