BD Sciences LSRII
The LSRII flow cytometer is capable of analyzing more labels than the Aria, but lacks sorting capability.
It is equipped with 355-, 405-, 488-, and 633-nm lasers to detect up to 11 fluorescent labels (2 off 355nm, 2 off 405nm, 5 off 488nm, and 2 off 633nm).
A digital acquisition system allows for inter- and intra-beam compensation as well as the ability to threshold any parameter.
Signals are digitized 10 million times per second in 16,384 discrete levels for superior channel resolution and sensitivity.
The system includes the High Throughput Sampler (HTS) option to handle 96- and 384-well microtiter plates to permit high-content, high-speed screening.
Rates and Fees
Internal Users:
$45/hr
$95/hr if full assistance is needed
$50/hr training
External Non-Profit Users:
$73/hr $153/hr if full assistance is needed $81/hr training
External Industry Users:
$145/hr $306/hr if full assistance is needed
$161/hr training
Online FACS training courses
FloJo FACS analysis software
BD fluorescence spectra viewer: a great resource for planning FACS experiments.
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