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Microscopy and Imaging Core Facility
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 Microscopy Home

 Policy for Use

 Online Scheduling

 Rates and Fees

 General Resources

Instructions for
Online Scheduling:

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BD Sciences LSRII

BD Sciences LSRII

The LSRII flow cytometer is capable of analyzing more labels than the Aria, but lacks sorting capability.

It is equipped with 355-, 405-, 488-, and 633-nm lasers to detect up to 11 fluorescent labels (2 off 355nm, 2 off 405nm, 5 off 488nm, and 2 off 633nm).

A digital acquisition system allows for inter- and intra-beam compensation as well as the ability to threshold any parameter.

Signals are digitized 10 million times per second in 16,384 discrete levels for superior channel resolution and sensitivity.

The system includes the High Throughput Sampler (HTS) option to handle 96- and 384-well microtiter plates to permit high-content, high-speed screening.

Rates and Fees

Internal Users:
$45/hr
$95/hr if full assistance is needed
$50/hr training

External Non-Profit Users:
$73/hr
$153/hr if full assistance is needed
$81/hr training

External Industry Users:
$145/hr
$306/hr if full assistance is needed
$161/hr training

Resources

Online FACS training courses

FloJo FACS analysis software

BD fluorescence spectra viewer: a great resource for planning FACS experiments.

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