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Microscopy and Imaging Core Facility
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 Microscopy Home

 Policy for Use

 Online Scheduling

 Rates and Fees

 General Resources

Instructions for
Online Scheduling:

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Amaxa Nucleofector

Amaxa Nucleofector

The Amaxa nucleofector uses a novel transfection technology to introduce nucleic acids into stem cells, primary cells, and cell lines that are difficult to transfect.

It employs a non-viral method based on an optimized combination of electrical parameters and cell-type specific solutions.

Both DNA and RNA are efficiently transfected into both the cytoplasm and nucleus of cells. Thus, the Amaxa provides the ability to transfect even non-dividing cells, such as neurons and resting blood cells.

In addition, the transfection efficiency is much higher than traditional methods can achieve. Experiments involving transfection of siRNA or RNAi, transient or stable gene expression, small-scale protein production are all possible.

The Amaxa is compatible with individual cuvettes as well as 96-well trays.

Rates and Fees

Internal Users:
$10/hr
$45/hr if full assistance is needed
$50/hr training

External Non-Profit Users:
$17/hr
$73/hr if full assistance is needed
$81/hr training

External Industry Users:
$33/hr
$145/hr if full assistance is needed
$161/hr training

Resources

Amaxa website

Cell database: great resource to determine optimal transfection settings for your favorite cells

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