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Analytical Biochemistry Core Facility Instrumentation
MicroCal VP-Isothermal Titration Calorimeter (VP-ITC)
Isothermal Titration Calorimetry allows a simultaneous determination of all binding parameters (equilibrium constant, enthalpy of binding and stoichiometry of the interaction) in a single experiment - information unobtainable from more limited binding assays.
Main Technical Features
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- The range of binding constants is broad: K from sub-millimolar to nanomolar (102 to 109 M-1).
- It is a true in-solution technique: No labeling or immobilization is required.
- Active cell volume ~ 1.4 ml.
- Non-reactive Hastelloy® cells for excellent chemical resistance.
- Fixed-in-place cells for reproducible ultrasensitive performance with low maintenance.
- Precision liquid delivery system for accurate and reproducible injections.
- Operating temperature range of 2ºC to 80ºC.
- Peltier controlled for rapid temperature equilibration.
- Includes ThermoVac® sample preparation and cleaning device.
- Includes Single Injection Method and ITC-Expert software.
Some Applications
- Characterization of molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules.
- Assessment of the effect of molecular structure changes on binding mechanisms.
- Assessment of biological activity.
Rates and Fees
Hourly rates. These rates are in effect from July 1, 2009 through June 30, 2010, and are subject to change without further notice.
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RPI Users
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External Not for Profit
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External Industry
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| Approved |
10
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16
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32
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| Assisted |
20
|
32
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64
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| Training |
50
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81
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161
|
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