A. Introduction

B. Chromatographic Fundamentals

 1. Retention
 2. Band Spreading
 3. Resolution

C. Dynamics of Chromatography

 1. Basic mass transfer equations
 2. Method of moments
 3. Linear dispersion model
 4. Linear staged models for chromatography

D. Instrument Requirements for Chromatography

 1. System design
 2. Column packing techniques

E. Fundamentals of Adsorption

 1. Gibbs adsorption isotherm
 2. Adsorption isotherm models
 3. Local equilibrium theory and solute movement plots

F. Modes of Chromatography

 1. Reversed phase and hydrophobic interaction
 2. Ion exchange
 3. Size-exclusion
 4. Group specific and biospecific affinity

G. Detectors in Liquid Chromatography

 1. UV-Vis
 2. Refractive Index
 3. Fluorescence
 4. Mass Spec.

H. Gradient Elution Chromatography

I. Preparative Chromatography

 1. Preparative elution
 2. Frontal
 3. Gradient
 4. Displacement chromatography
 5. Optimization

J. Novel Chromatographic Morphologies

 1. Continuous annular systems
 2. Radial flow
 3. Perfusion chromatography
 4. Membrane chromatography

K. Chromatographic Applications in Biotechnology

1. Applications of various modes of operation
2. Sequencing of chromatographic operations
3. Multidimensional separations for proteomics