The following table will provide you with
the recognition sequences for some of the most
frequently used restriction endonucleases. The sequences given are of one
strand, in the
5' to 3' direction.
|
Enzyme |
Organism |
Recognition Sequence |
Blunt or Sticky End |
|
EcoRI |
Escherichia Coli |
GAATTC |
Sticky |
|
BamHI |
Bacillus amyloliquefaciens |
GFATCC |
Sticky |
|
BglII |
Bacillus globigii |
AGATCT |
Sticky |
|
PvuI |
Proteus vulgaris |
CGATCG |
Sticky |
|
PvuII |
Proteus vulgaris |
CAGCTG |
Blunt |
|
HindIII |
Haemophilus influenzae Rd |
AAGCTT |
Sticky |
|
Hinfl |
Haemophilus influenzae Rf |
GANTC |
Sticky |
|
Sau3A |
Staphylococcus aureus |
GATC |
Sticky |
|
AluI |
Arthrobacter luteus |
AGCT |
Blunt |
|
TaqI |
Thermus aquaticus |
TCGA |
Sticky |
|
HaeIII |
Haemophilus aegyptius |
GGCC |
Blunt |
|
NotI |
Nocardia otitidis-caviarum |
GCGGCCGC |
Sticky |
This table was taken from "Gene Cloning an introduction" (3rd
Edition)
written by T.A. Brown and published by Stanley Thornes Ltd.
Back to Recombinant DNA
Created by Matthew Kuure-Kinsey and Beth McCooey for Biochemical Engineering Fall 2000