Recognition Sequences



The following table will provide you with the recognition sequences for some of the most
frequently used restriction endonucleases. The sequences given are of one strand, in the
5' to 3' direction.

Enzyme

Organism

Recognition Sequence

Blunt or Sticky End

EcoRI

Escherichia Coli

GAATTC

Sticky

BamHI

Bacillus amyloliquefaciens

GFATCC

Sticky

BglII

Bacillus globigii

AGATCT

Sticky

PvuI

Proteus vulgaris

CGATCG

Sticky

PvuII

Proteus vulgaris

CAGCTG

Blunt

HindIII

Haemophilus influenzae Rd

AAGCTT

Sticky

Hinfl

Haemophilus influenzae Rf

GANTC

Sticky

Sau3A

Staphylococcus aureus

GATC

Sticky

AluI

Arthrobacter luteus

AGCT

Blunt

TaqI

Thermus aquaticus

TCGA

Sticky

HaeIII

Haemophilus aegyptius

GGCC

Blunt

NotI

Nocardia otitidis-caviarum

GCGGCCGC

Sticky



This table was taken from "Gene Cloning an introduction" (3rd Edition)
written by T.A. Brown and published by Stanley Thornes Ltd.



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Created by Matthew Kuure-Kinsey and Beth McCooey for Biochemical Engineering Fall 2000