HELP GUIDE
Biliproteins are light-harvesting and excitation energy transfer pigments, which transfer excitons to photosystem II. Excess energy can be redistributed from photosystem II to photosystem I by a process called spillover. They can be found in three types of organisms: cryunobacteria (blue-green algae), red algae, cryptomonads.
Chromophores contain peptides that were produced by trypsin treatment and purified in order to isolate the individual peptide-bound bilins free of overlapping absorption. Chromophores are things or materials that give proteins color.
Phycoerythrobilins are a form of biliprotein and the term 545 means that the absorption peak for this protein is at 545 nm.
Cryptomonads are the genius from where the proteins are extracted from and in this case Rhodomonas lens.
Cryptobilins are species containing biliproteins which basically means that it has chromophores.
Circular dichroism and absorption spectroscopy in the visible region together with various biochemical protocols have been used to study these chromophores. The CD spectrum exhibits overlapping positive and negative bands. Exciton splitting between these closely-spaced pairs of chromophores produces a CD spectrum that has a positve and negative bands of equal rotational strengths, a conservative spectrum. Alternatively, any positive or negative band could arise from a single chromophore. The result of this study demonstrate that exciton splitting is the likely cause of the negative and corresponding positive bands.