<b><Center>Bioaugmentation Procedures

Introduction to Bioaugmentation

        In industry, often the biomass present needs reinforcements to increase its degradation efficiency. A method currently in use is bioaugmentaion, which adds microorganisms that have been isolated and selectively adapted to degrade specific compounds to existing biomass (Huban, Plowman, 74). The benefit of this method is that the microorganisms added are able to degrade contaminants not broken down by biomass already present in the pond, resulting in improved treatment of wastewater. Bioaugmentation changes the species composition of existing biomass by adding bacteria from natural sources or environmental isolates. Many natural bacteria can be found in the contaminated soils and water of sludge storage areas and lagoons. (75) For example, if bacteria was needed to degrade aliphatic hydrocarbons, a likely source would be storage area for the sludge produced in the degradation process. Example of Metabolic Pathway



Methodology

        The samples taken from storage facilities contain many different strains of bacteria, so the particular strain needed must be isolated. The method of isolation is to create an environment containing the organic compunds of interest and see which strains are able to survive and grow in their presence. After the candidate strains are isolated, they are tested to see which strains in the processing of degradation consume the most oxygen, oxidize the target compound, and produce the largest quantities of gas. (Huban, Plowman, 75). The next phase of theprocess is to remove minor strains to increase overall efficiency. From the sample strains remaining, the concentration of the target compound can be increased or more of these strains can be isolated to obtain the desired activity. A concern with bioaugmentation is the saprophytic bacteria, which is found in the majority of the stains isolated. Some of the bacteria, under certain conditions, are pathogens that can cause infection (76). The safety method used to combat this problem is to subject the stains to antibiotics, to see if these remedies will work.