INTRODUCTION TO BIOCHEMICAL ENGINEERING, 52:108, FALL 1996

 

Instructor: Jonathan S. Dordick, 129 CB, 335-1414

Several guest lectures will be presented. A featured guest lecturer is Professor Young Je Yoo from Seoul National University in Korea. Several other lectures will be presented by experts in specific topics of enzyme and fermentation technology.

Time and Place: MWF 10:30-11:20, 408 CB.

Office Hours: MW 9:00-10:00 a.m. or by appointment.

Teaching Assistant: Salvatore Attaguille plus one more graduate student to be announced.

Course Content: This is an introductory course on the principles of Biochemical Engineering. It is meant for a student with little or no formal training in the life sciences, and will focus on the engineering aspects of biotechnology. Such issues include enzyme technology, cell growth and product formation, transport in bioreactors, bioreactor design, media formulation and sterilization, bioseparations, and bioprocess economics.

Course Materials: The textbook for the course is by Blanch, H. W. and Clark, D. S., "Biochemical Engineering". The book can be purchased from the IMU Bookstore.

Suggested Supplemental Reading:

1. L. Stryer, "Biochemistry", 3rd Edition, W. H. Freeman & Co., New York.

2. C. K. Mathews and K. E. van Holde, "Biochemistry", Benjamin/Cummings Publishing Co., Redwood City, CA.

3. B. Alberts et al., "Molecular Biology of the Cell", Garland Publishing, Inc., New York.

4. R. Y. Stainer et al., "Introduction to the Microbial World", Prentice-Hall, Inc., Englewood Cliffs, NJ.

5. R. H. Abeles, P. A. Frey, and W. P. Jencks, "Biochemistry", Jones and Bartlett Publishers, Boston.

6. S. F. Aiba et al., "Biochemical Engineering", 2nd Edition, Academic Press, New York.

7. Enzyme Engineering - A series of 12 volumes.

Course Syllabus:

Lecture

Number

Date

Topic

Assignments

1

M-8/26

Introduction to Biochemical Engineering

Handout

   

Part A. Enzyme Technology

 

2

W-8/28

Proteins and Enzymes a primer

FUNDAMNT\enzymes.htm

3

F-8/30

Industrial Applications of Enzymes

Handout

 

M-9/2

No Class - Labor Day

 

4

W-9/4

Principles of Catalysis

pp. 1-5; 18-27

5

F-9/6

Introduction to Enzyme Kinetics - Single Substrate Kinetics

pp. 5-18; 50-52

IMMOB\enzkin.html

6

M-9/9

Graphical Representation of Kinetic Data

 

7

W-9/11

Enzyme Inhibition

pp. 39-45

8

F-9/13

Enzyme Stability

pp. 77-81

9

M-9/16

Introduction to Immobilized Enzyme Technology

pp. 103-116 IMMOB\whatis.htm

10

W-9/18

External Mass Transfer in Immobilized Enzyme Systems

pp. 116-127

11

F-9/20

Internal Mass Transfer in Immobilized Enzyme Systems

pp. 127-137

12

M-9/23

Internal Mass Transfer in Immobilized Enzyme Systems, Continued

 
   

Part B. Microbiology

 

13

W-9/25

Basic Microbiology

Handouts FUNDAMNT\cultures.htm

FUNDAMNT\micro.htm

 

Th-9/26

Exam Number 1, 7-9 pm

Includes Lectures 1-12

 

14

F-9/27

Cellular Chemistry and Taxonomy

Handouts

15

M-9/30

Quantitative Aspects of Cell Growth

pp. 162-171

16

W-10/2

Introduction to Metabolism

Handouts

FUNDAMNT\energy.htm

17

F-10/4

Glycolysis and the Citric Acid Cycle

Handouts

   

Part C. Microbial Cultivation

 

18

M-10/7

Batch Culturing of Microorganisms

pp. 276-280

FERMENT\batchb.htm

19

W-10/9

Fermentation Yields

pp. 173-181

20

F-10/11

Cell Growth Models

pp. 181-209 FERMENT\limiting.htm

21

M-10/14

Design of Fermentation Media

FERMENT\games.htm

22

W-10/16

Introduction to Continuous Culture

pp. 280-291

Contin\working.htm

23

F-10/18

Enzymatic Catalysis in Continuous Culture; Multi-Stage Continuous Culture

pp. 292-297

24

M-10/21

Continuous Culture and Recycle; Fed-Batch

pp. 305-308; 322-328

25

W-10/23

Problem Solving

 
 

Th-10/24

Exam Number 2, 7-9 pm

Includes Lectures 13-25

 
   

Part D. Bioprocessing

 

26

F-10/25

Introduction to Bioprocessing; Media Sterilization

pp. 415-426

FERMENT\sterint.htm

27

M-10/28

Introduction to Oxygen Transfer in Biological Reactors

p. 343; pp. 353-361

bchemprj4.html

28

W-10/30

Oxygen Transfer in Biological Reactors, Continued

pp. 390-415

29

F-11/1

Oxygen Transfer in Biological Reactors, Continued

 

30

M-11/4

Introduction to Fermentor Design

AERATION\mix\scale.htm

31

W-11/6

Scale-Up of Bioreactors

 

32

F-11/8

Problem Solving

 

33

M-11/11

Overview of Downstream Processing (Bioseparations); Centrifugation

pp. 453-467

DOWNSTREAM\fixed.htm

34

W-11/13

Filtration and Ultrafiltration

pp. 467-470; 490

FILTRATION\guide.htm

Membranes\intro.htm

35

F-11/15

Precipitation of Proteins

pp. 491-502

PRECIP/Enzpurif.htm

PRECIP\precpintro.html

36

M-11/18

Chromatography

pp. 502-511

CHROMO\chromintro.html

37

W-11/20

Electrophoresis and Crystallization

pp. 533-542

DOWNSTREAM\electrop.htm

DOWNSTREAM\crystal.htm

38

F-11/22

Review

 
   

Part E. Genetic Engineering

 

39

M-11/25

Molecular Genetics and Recombinant DNA Technology

Handouts

FUNDAMNT\genetics.htm

 

W-11/27

No Class - Thanksgiving

 
 

F-11/29

No Class - Thanksgiving

 

40

M-12/2

Site-Directed Mutagenesis

Handouts

   

Part F. Industrial Bioprocessing and Process Economics

 

41

W-12/4

Manufacture of Biological Products

pp. 609-643

42

F-12/6

Economic Analysis of Bioprocesses

pp. 643-677

43

M-12/9

Economic Analysis of Bioprocesses, Continued

 

44

W-12/11

Careers in Biotechnology

 

44

F-12/13

Review

 

 

M-12/16

Exam Number 3 (2:15 p.m. - Exam Week)

Includes Lectures 26-43

 

 

Grading: The course grading will consist of three parts. Part 1 are the three exams. These exams are not cumulative and will test knowledge gained through the indicated lectures. I will be the sole grader of the exams. Part 2 is homework which will be due periodically throughout the semester. The homework will be prepared by me, yet graded solely by the TAs. I will allow study groups to discuss homework assignments; however, the homework that you submit must be your own. Part 3 is in-class participation. I admit that this is somewhat subjective, but I want all students to participate in discussions, problem solving, and asking questions. The following is a breakdown of the grading:

Exams 75%

Homework 20%

Participation 5%