Proteins synthesized in genetically engineered organisms and intended for injection into animals must be stringently purified. Pyrogens from E. coli, including the outer envelope lipopolysaccharide (LPS) must be removed or inactivated. Hence product recovery operations such as affinity chromatography are an important step in the manufacturing process.
Given below is a schematic representation
of the typical steps involved in processing human leukocyte interferon
produced by recombinant DNA techniques. This will give you an idea of where
exactly affinity chromatography is usually involved in the realm of bioprocessing.
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