Now let us look at a specific example where affinity chromatography is used, namely, in the purification of human leukocyte interferon made in E. coli.

Proteins synthesized in genetically engineered organisms and intended for injection into animals must be stringently purified. Pyrogens from E. coli, including the outer envelope lipopolysaccharide (LPS) must be removed or inactivated. Hence product recovery operations such as affinity chromatography are an important step in the manufacturing process.

Given below is a schematic representation of the typical steps involved in processing human leukocyte interferon produced by recombinant DNA techniques. This will give you an idea of where exactly affinity chromatography is usually involved in the realm of bioprocessing.
 

SO, Thank you everybody, for reading so patiently to what I had to say about affinity chromatography....

I hope you enjoyed the show...


I want to go HOME now :-)

Why not try more Chromatography...