4. The result of affinity chromatography

Fig. 6 . The result of affinity chromatography

The components can be eluted by the solvent at the sequence of affinity to the ligand. The component, which has the weakest affinity with the ligands, will be eluted first. For example, as shown in figure 6, these components can be ranked by the affinity as:

Component affinity:  3<2<1

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