C H R O M A T O G R A P H Y


In any chemical or bioprocessing industry, the need to separate and purify a product from a complex mixture is a necessary and important step in the production line. Today, there exists a wide market of methods in which industries can accomplish these goals. One method that has been around for quite some time and is used in many industries is chromatography.

Chromatography is the process in which a complex mixture is eluted into a column, where afterwards, the different components of the mixture emerge separately. This works on the principle that molecules in a mixture are separated based on their individual affinities for some adsorptive material, causing differential rates of migration over these materials. The smaller the affinity a molecule has, shorter the time spent in a column. With this in mind, it is easy to understand how different components will move at different rates through a column, emerging at specific points in time.

Introduction to the process

The chromatogram

Scale-up of the column

Types of chromatography

Credits and acknowledgements


This web page is designed to present a brief introduction to the basics of chromatographic separations. Furthermore, the information presented is complementary to the Biochemical Engineering Course at Rensselaer Polytechnic Institute.

© 1997 Kevin Yip - Introduction to Biochemical Engineering

This page last updated by Kevin Yip on May 1, 1997