There are quite a few enzymes which could be used as glucose detectors. Many of them
require cofactors, though. The following example shows the glucose enzyme reaction with glucose
oxidase. This enzyme doesn't require cofactors and is in addition one of the most stable enzymes for
With the reaction shown above there are two possibilities to measure the glucose
concentration: either one measures the oxygen consumption or the
hydrogen-peroxide production. As a third option it would be also possible to
measure the temperature increase due to heat released by the two reactions.
The presence of the second enzyme (catalase)a although not really essential for the assay, enhances the first reaction. This happens because of the decomposition of the hydrogen peroxide.
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