Wave Biotech
A very simple method used by Wave Biotech LLC, 999 Frontier Road, Bridgewater, New Jersey, 08807 achieves gentle agitation of plastic bag bioreactors. The bags are placed on a rocker resembling a seesaw. This method is particularly suitable for cells that require little or no aeration.
The Wave apparatus is becoming popular. It could soon be the standard against which other systems are compared.
From their web page:
"Proven for animal, virus, insect, and plant cell culture in suspension, or on carriers. The Wave Bioreactor is the ideal device for cell culture. Culture medium and cells only contact a presterile, disposable chamber called a Cellbag that is placed on a special rocking platform. The rocking motion of this platform induces waves in the culture fluid. These waves provide mixing and oxygen transfer, resulting in a perfect environment for cell growth that can easily support over 20 x 106 cells/ml. The bioreactor requires no cleaning or sterilization, providing the ultimate ease in operation and protection against cross-contamination."
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