Let's consider the output from the detector: the chromatogram. The figure below will help you to follow along in our discussion.
Since the sample is separated in the column, different peaks on the chromatogram correspond to different components in the sample mixture. The chromatograms above show the results of separations of protein mixtures by ion exchange chromatography. The lettered peaks correspond to different proteins (A = ovalbumin, B = conalbumin, C = cytochrome c, D = lysozyme). The separation corresponding to the chromatogram on the left was performed at pH 5.85, while the one on the right was performed at pH 6.5. It is evident that operation conditions such as pH and temperature have a significant effect on the output.
We have completed another segment of our journey! Have fun considering different types of chromatography,.
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