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Rensselaer Polytechnic Institute Department of Biological Sciences
Mattheos Koffas
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Department of Biological Sciences
1W14 Jonsson-Rowland Science Center
Rensselaer Polytechnic Institute
110 Eighth Street
Troy, NY 12180-3590

Phone: (518) 276-6446
Fax: (518) 276-2344

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Biology Home Undergraduate Graduate Faculty Research News and Events Contacts
Mattheos Koffas

Associate Professor of Biology

Education and Training

Ph.D. (Biochemical Engineering) Massachusetts Institute of Technology
Diploma in Engineering, National Technical University, Athens, Greece

Contact

E-mail: koffam@rpi.edu
Tel: (518) 276-2220

Rensselaer Polytechnic Institute
110 8th Street
Troy, NY 12180

Research Interests

The central theme of Koffas' research is metabolic engineering of unicellular organisms with the purpose of efficiently producing high value chemicals, such as nutraceuticals and pharmaceuticals, and proteins of therapeutic importance. Within this context, several different approaches are applied, including 1) development of computational tools to enable the prediction of genetic modifications that would result in increasing the flux in a pathway of interest; 2) protein engineering to allow the functional expression of eukaryotic enzymes in simple microorganisms; and 3) identification of previously unknown metabolic enzymes, with emphasis on enzymes involved in targeted hydroxylations and glycosylations; (4) development of metabolic switches for the dynamic control of metabolic fluxes, based on the concentration of intracellular metabolites.

Selected Publications

Wang, W.; Englaender, J.A.; Xu, P.; Mehta, K.K.; Suwan, J.; Dordick, J.; Zhang, F.; Yuan, Q.; Linhardt, R.J.; Koffas, M.A.G. “Expression of low endotoxin 3-O-sulfotransferase in Bacillus subtilis and Bacillus megateriumApplied Biochemistry and Biotechnology August 3rd 201

Xu, P.; Gu. Q.; Wang, W.; Wong, L.; Bower, A.; Collins, C.; Koffas, M.A.G.; “Modular optimization of multi-gene pathways for fatty acids production in E.coliNature Communications 2013 4:1409

Mora-Pale, M.; Linhardt, R.; Dordick, J.; Koffas, M.A.G.; “ Metabolic engineering and in vitro biosynthesis of phytochemicals and non-natural analogues” Plant Science 2013 Sep;210:10-24

Bhan, N.; Xu, P.; Koffas, M.A.G.; “Pathway and protein engineering approaches to produce novel and commodity small molecules” Current Opinion in Biotechnology 2013; March  13

Cress, B.; Linhardt, R.; Koffas, M.A.G.; “Draft Genome Sequence of Escherichia coli Strain Nissle 1917 (Serovar O6:K5:H1)” Genome Announcements, 2013 Feb 28; 1(2);

Xu, P.; Bhan, N.; Koffas, M.A.G.; “Engineering plant metabolism into microbes: from systems biology to synthetic biology” Current Opinion in Biotechnology, 2012, Sept. 14.

Bhan, N.; Xu, P.; Khalidi, O.; Koffas, M.A.G.; “Redirecting carbon flux into malonyl-CoA to improve resveratrol titers: Proof of concept for genetic interventions predicted by OptForce computational framework” Chemical Engineering Science, [epub ahead of print]

Xu, P.; Vansiri, A.; Bhan, N.; Koffas, M.A.G.; “ePathBrick: a synthetic biology platform for engineering metabolic pathways in E.coliACS Synthetic Biology (2012), 1(7), 256-266.

Malla, S.; Koffas, M.A.G.; Kazlauskas, R.J.; Kim, B.G. “Production of 7-O-methyl aromadendrin, a medicinally valuable flavonoid, in Escherichia coli” Applied and Environmental Microbiology. 2012 Feb; 78(3): 684-94.

Fowler, Z.L.; Shah, K.; Panepinto, J.C.; Jacobs, A.; Koffas, M.A.G. “Development of non-natural flavanones as antimicrobial agents” PLoS One 2011; 6(10): e25681.

Xu, P.; Ranganathan, S.; Fowler, Z.L.; Maranas, C.D.; Koffas, M.A.G. “Genome-scale metabolic modeling results in minimal interventions that cooperatively force carbon flux towards malonyl-CoA” Metabolic Engineering, 2011 Sep; 13(5):578-87.

Lim, G.-C.; Fowler, Z.L.; Hueller T.; Schaffer S.; Koffas MAG.”High-yield resveratrol production in engineered Escherichia coliApplied and Environmental Microbiology, 2011 May; 77(10):3451-60.

Fowler, Z.; Baron, C.; Panepinto, J.; Koffas, M.A.G. “Melanization of flavonoids by fungal and bacterial laccases” Yeast, 2011 Mar; 28(3); 181-188.

Santos C.M.; Koffas M.A.G.; Stephanopoulos, G. ; “Optimization of a heterologous pathway for the production of flavonoids from glucose” Metabolic Engineering, 2011 Jul; 13(4):392-400.

Chemler, J.A.; Lim, C.-G.; Daiss, J.L.; Koffas, M.A.G.; “A versatile microbial system for biosynthesis of novel polyphenols with altered estrogen receptor binding activity” Chemistry & Biology, 2010 Apr 23;17(4):392-401

Xu, P.; Koffas, M.A.G.; “Use of E. coli for the synthesis of altenrative fuels” Biofuels May 2010, Vol. 1, No. 3, Pages 493-504.

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Rensselaer Polytechnic Institute Department of Biological Sciences
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