Interactive Quiz on
Microtubules
Reading:
Textbook Reading: Alberts et al. 4th Edition Chapter 16. Specific pages given in the notes.
Some recent articles
(optional reading):
J. Löwe, H. Li, K. H. Downing & E. Nogales
(2001) "Refined structure of ab-tubulin at 3.5
Å resolution," J. Mol. Biol. 313: 1045-1057.
S. I. Dutcher (2001) "The tubulin fraternity: alpha to eta," Curr. Opin. Cell
Biol. 13: 49-54.
M. Moritz & D. A. Agard (2001) "g-Tubulin complexes
and microtubule nucleation," Cur. Opin. Struct. Biol. 11: 174-181.
C. L. Rieder, S. Faruki & A. Khodjakov (2001) "The centrosome in vertebrates:
more than a microtubule-organizing center," Trends in Cell Biol. 11: 413-419.
K. Kinoshita, B. Habermann & A. A. Hyman (2002) "XMAP215: a key component of the
dynamic microtubule cytoskeleton," Trends in Cell Biol: 12: 267-273.
S. G. Addinall & B. Holland (2002) "The tubulin ancester, FtsZ, draughtsman,
designer and driving force for bacterial cytokinesis," J. Mol. Biol. 318:
219-236.
M. Bornens (2002) "Centrosome composition and microtubule anchoring mechanisms,"
Current Opinion in Cell Biol. 14: 25-34.
D. Job, O. Valiron & B. Oakley (2003) "Microtubule nucleation," Current Opinion
in Cell Biol. 15: 111-117.
JA. Dammermann, A. Desai & K. Oegema (2003) "The minus end in sight," Current
Biol. 13: R614-R624.
J. Howard & A. A. Hyman (2003) "Dynamics and mechanics of the microtubule plus
end," Nature 422: 753-758.
J. Beisson & M. Wright (2003) "Basal body/centriole assembly and continuity,"
Current Opinion in Cell Biol. 15: 96-104.
Y. Ou, M. Zhang & J. B. Rattner (2004) "The centrosome: the centriole-PCM
coalition," Cell Motility & Cytoskeleton 57: 1-7.
G. C. Rogers, S. L. Rogers & D. J. Sharp (2005) "Spindle microtubules in flux,"
J. Cell Sci. 118: 1105-1116.
X. Xiang (2006) "A + TIP for a smooth trip," J. Cell Biol. 172: 651-654.
X. Wu, X. Xiang & J. A. Hammer III (2006) "Motor proteins at the microtubule
plus-end," TRENDS in Cell Biol 16: 135-143.
C. Wiese & Y. Zheng (2006) "Microtubule nucleation: g-tubulin
and beyond," J. Cell Sci. 119: 4143-4153.
1.a. Describe the structure and composition
of an individual microtubule. What is the basic unit that adds or dissociates
during lengthening or shortening of a microtubule? What nucleotides are
associated with these units, and where are they located within the molecule?
What is the fate of the nucleotides after polymerization? What is thought to function as
GTPase activating protein (GAP)?
b. Describe and explain the dynamic instability exhibited by some microtubules
at their plus end.
c. Summarize the composition and role of the g-tubulin
ring complex.
Copyright © 1999-2006 by Joyce J. Diwan. All rights reserved.
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