Biochemistry of Metabolism: Cell Biology

Microtubule Motor Proteins

Reading:

Textbook Reading: Alberts et al. 4th Edition Chapter 16. Specific pages given in the notes.

Some recent articles (optional reading): 
R. L. Karcher, S. W. Deacon & V. I Gelfand (2002) "Motor-cargo interactions: the key to transport specificity," Trends in Cell Biol. 12: 21-27.
F. J. Kull & S. A. Endow (2002) "Kinesin: switch I & II and the motor mechanism," J. Cell Science 115: 15-23.
E. Mandelkow & E.-M. Mandelkow (2002) "Kinesin motors and disease," Trends in Cell Biol. 12: 585-591.
A. Kamal & L. S. B. Goldstein (2002) "Principles of cargo attachment to cytoplasmic motor proteins," Current Opinion in Cell Biol. 14: 63-68.
D. L. Dujardin & R. B. Vallee (2002) "Dynein at the cortex," Curr. Opinion in Cell Biol. 14: 44-49.
J. L. Rosenbaum & G. B. Witman (2002) "Intraflagellar transport," Nature Rev. Molec. Cell Biol. 3: 813-825.
G. J. Pazour & J. L. Rosenbaum (2002) "Intraflagellar transport and cilia-dependent diseases," Trends in Cell Biol: 12: 551-555.
S. A. Endow & D. S. Barker (2003) "Processive and nonprocessive models of kinesin movement," Annu. Rev. Physiol. 65: 161-175.
J. M. Scholey (2003) "Intraflagellar transport," Annu. Rev. Cell Dev. Biol. 19: 423-443.
S. M. King (2003) "Organization and regulation of the dynein microtubule motor," Cell Biol. Internat. 27: 213-215.
S. P. Gross (2003) "Dynactin: coordinating motors with opposite inclinations," Current Biol. 13: R320-R322.
T. A. Schroer (2004) "Dynactin," Annu. Rev. Cell Dev. Biol. 20: 759-779.
E. P. Sablin & R. J. Fletterick (2004) "Coordination between motor domains in processive kinesins," J. Biol. Chem. 279: 15707-15710.
C. L. Asbury, A. N. Fehr & S. M. Block (2003) "Kinesin moves by an asymmetric hand-over-hand mechanism," Science 302: 2130-2134.
R. A. Cross (2004) "The kinetic mechanism of kinesin," Trends in Biochem. Sci. 29: 301-309.
M. J. Wozniak, R. Milner & V. Allan (2004) "N-Terminal kinesins: many and various," Traffic 5: 400-410.
M. Sakato & S. M. King (2004) "Design and regulation of the AAA+ microtubule motor dynein," J. Struct. Biol. 146: 58-71.
M. A. Welte (2004) "Bidirectional transport along microtubules," Current Biol. 14: R525-R537.
G. C. Rogers, S. L. Rogers & D. J. Sharp (2005) "Spindle microtubules in flux," J. Cell Sci. 118: 1105-1116.
P. J. Hollenbeck & W. M. Saxton (2005) "The axonal transport of mitochondria," J. Cell Sci. 118: 5411-5419.

Potential Test Question:

1. a. Diagram and describe the structure of kinesin I (conventional kinesin), and indicate the roles of different parts of the kinesin molecule. Describe and explain the significance of the conformational change associated with phosphorylation/dephosphorylation of the kinesin light chains or binding and unbinding of cargo.
b. What is meant by the statement that movement of kinesin I along a microtubule is processive? How is a processive mechanism of movement suited to the role of kinesin?
c. Briefly describe the structure and composition of the motor domain of dynein. Briefly describe two ways in which the dynein motor domain differs from that of kinesin.

Copyright 1999-2006 by Joyce J. Diwan. All rights reserved.

Lecture Notes on
Microtubule Motors

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   Microtubule Motors      

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