Molecular Biochemistry II

Photosynthetic Dark Reaction - Calvin Cycle


Textbook Reading: Voet & Voet, Biochemistry, 3rd Edition, p. 896-905.

Some recent articles (optional reading): 
S. J. Crafts-Bradner & M. E. Salvucci (2000) "Rubisco activase constrains the photosynthetic potential of leaves at high temperature and CO2," PNAS 97: 13430-13435. (See also commentary by R. G. Jensen, p. 12937-12938.)
M. Matsuoka, R. T. Furbank, H. Fukayama & M. Miyao (2001) "Molecular engineering of C4 photosynthesis," Annu. Rev. Plant Physiol. Plant Mol. Biol. 52: 297-314.
R. E. Husler, H.-J. Hirsch, F. Kreuzaler & C. Peterhnsel (2002) "Overexpression of C4-cycle enzymes in transgenic C3 plants: a biotechnological approach to improve C3-photosynthesis," J. Exptl. Botany 53: 591-607.
I. Andersson & T. C. Taylor (2003) "Structural framework for catalysis and regulation in ribulose-1,5-bisphosphate carboxylase/oxygenase," Arch. Biochem. Biophys. 414: 130-140.
R. J. Spreitzer (2003) "Role of the small subunit in ribulose-1,5-bisphosphate caboxylase/oxygenase," Arch. Biochem. Biophys. 414: 141-149.
P. Westhoff & U. Gowik (2004) "Evolution of C4 phosphoenolpyruvate carboxylase. Genes and proteins: a case study with the genus Flaveria," Ann. Botany 93: 13-23.
B. B. Buchanan & Y. Balmer (2005) "Redox regulation: a broadening horizon," Annu. Rev. Plant Biol. 56: 187-220.
L. Cegelski & J. Schaefer (2006) "NMR determination of photorespiration in intact leaves using in vivo 13CO2 labeling," J. Magnetic Res. 178: 1-10.
L. E. Anderson, J. Chrostowski & A. A. Carol (2006) "Enzyme co-localization with transketolase, xylulose-5-P 3-epimerase and phosphoriboisomerase in pea leaf chloroplasts," Plant Science.
S. Reumann & A. P. M. Weber (2006) "Plant peroxisomes respire in the light: Some gaps of the photorespiratory C2 cycle have become filled - Others remain," Biochim. Biophys. Acta 1763: 1496-1510.

Potential Test Question:

1. a. Write out the reaction catalyzed by RuBP Carboxylase, giving names and structures of substrates, intermediates and products.
    b. Describe the subunit structure of the enzyme RuBP Carboxylase. Describe the location and nature of active site domains. Diagram and describe the reaction by which a carbamate group forms at the active site. What is its role?  
    c. In what ways does light affect RuBP Carboxylase activity? Does the "dark reaction" occur in the dark?

Copyright 1999-2007 by Joyce J. Diwan. All rights reserved.

Lecture Notes on
Photosynthetic Dark Reaction

Interactive Quiz on        
   Photosynthetic Dark Reaction

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