11S-20S-11S Complex

PDB file 1FNT

This file depicts the structure of the yeast 20S proteasome complexed with the 11S regulatory cap of Trypanosoma brucei. Crystal structure solved by F. G. Whitby, E. Masters, L. Kramer, J. R. Knowlton, Y. Yao, C. C. Wang & C. P. Hill in 2000.

Suggested display options:

Color chain to distinguish the different polypeptides. (Note that Chime does not have enough colors to give each of the 42 polypeptides a unique color.)
Try displays of cartoons and spacefill.
Drag to view the core complex and 11S caps and their interaction.

Now select protein and display as cartoons.
Use the command line to select *a, *b, *c, *d, *e, *f, *g.
Change display to spacefill.
Examine the end of the proteasome core complex.
Question: How does the end of the proteasome core complex compare to what you observed for the core complex crystallized in the absence of a cap?

To separately examine the structure of the cap, select protein and display as spacefill.
Now use the command line to select *a, *b, *c, *d, *e, *f, *g and use the mouse to select hide.
Note that Chime will not allow you to separately select subunits of the cap complex, because the number of subunits exceeds the capacity of the software.


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