What subdomains of actin are bound by gelsolin?
How is this consistent with the role of gelsolin?
What type of residues provide ligands to the other Ca++ in gelsolin?
How do the Ca++ binding sites compare to those in calmodulin?
Gelsolin-Actin
PDB file 1DBO
This file depicts a complex of G-Actin with the C-terminal half of gelsolin, with bound ATP & Ca++. The structure was solved by R. C. Robinson, M. Mejillano, V. P. Le, L. D. Burtnick, H. L. Yin, & S. Choe in 1999.
Suggested display options:
Color the protein chain
and display as cartoon.
Select
hetero-ligand,
display as spacefill, and
color CPK.
There are two Ca++ binding sites, plus Ca++ is substituting for Mg++
at the nucleotide-binding site of actin.
What is the secondary structure at each Ca++ binding site?
Residues of one Ca++ binding site may be selected as glu167a,
asp487b, gly492b, pro494b (Chain a is actin; chain b is gelsolin.) Display as ball
& stick and color CPK.
What atoms of gelsolin and actin interact with Ca++?
Questions:
What subdomains of actin are bound by
gelsolin?
How is this consistent with the role of gelsolin?
What type of residues provide
ligands to the other Ca++ in gelsolin?
How do the Ca++ binding sites compare to those in
calmodulin?
Return to Lecture
Notes
on Actin
Or press Back button
at top twice
to return to your place in the notes.