Molecular Biochemistry I

Oxidative Phosphorylation: F₁F_o ATPase

Reading:

Textbook Reading: Voet & Voet, Chapter 22, especially p. 827-833.

Some recent articles (optional reading): 
R. A. Capaldi & R. Aggeler (2002) "Mechanism of the F₁F_o-type of ATP synthase, a biological rotary motor," Trends in Biochem. Sci. 27: 154-160.
P. D. Boyer (2002) "A research journey with ATP Synthase," J. Biol. Chem. 277: 39045-39061.
J. Weber & A. E. Senior (2003) "ATP synthesis driven by proton transport in F₁F_o-ATP synthase," FEBS Lett. 545: 61-70.
G. Oster & H. Wang (2003) "Rotary protein motors," Trends in Cell Biol. 13: 114-121.
R. H. Fillingame, C. M. Angevine & O. Y. Dmitriev (2003) "Mechanics of coupling proton movements to c-ring rotation in ATP synthase," FEBS Lett. 555: 29-34.
M. Karplus & Y. Q. Gao (2004) "Biomolecular motors: the F₁-ATPase paradigm," Curr. Opinion in Struct. Biol. 14: 250-259.
K. Kinosita, K. Adachi & H. Itoh (2004) "Rotation of F₁-ATPase: how an ATP-driven molecular machine may work," Annu. Rev. Biophys. Biomol. Struct. 33: 245-268.
C. Chen, Y. Ko, M. Delannoy, S. J. Ludtke, W. Chiu & P. L. Pedersen (2004) "Mitochondrial ATP Synthasome," J. Biol. Chem. 279: 31761-31768.
R. Kagawa, M. G. Montgomery, K. Braig, A. G. W. Leslie & J. E. Walker (2004) "The structure of bovine F₁-ATPase inhibited by ADP and beryllium fluoride," EMBO J. 23: 2734-2744.
P. Falson, A. Goffeau, M. Boutry & J.-M. Jault (2004) "Structural insight into the cooperativity between catalytic and noncatalytic sites of F1-ATPase," Biochim. Biophys. Acta 1658: 133-140.
J. E. Walker & V. K. Dickson (2006) "The peripheral stalk of the mitochondrial ATP synthase," Biochim. Biophys. Acta 1757: 286-296.
J. Weber (2006) "ATP synthase: Subunit-subunit interactions in the stator stalk," Biochim. Biophys. Acta 1757: 1162-1170.
B. A. Feniouk, T. Suzuki & M. Yoshida (2006) "The role of subunit epsilon in the catalysis and regulation of F_oF₁-ATP synthase," Biochim. Biophys. Acta 1757: 326-338.

Potential Test Question:

Diagram and describe major features of the "Binding Change Mechanism" that has been proposed for the F₁F_o ATPase.
How does the crystal structure of the F₁ ATPase that you explored with Chime support this concept? What subunits of the E. coli F₁F_o complex constitute the stator and what parts the rotor of this molecular motor?
Describe the experiment that confirmed the rotational mechanism.

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Lecture notes on F1Fo ATP Synthase

Interactive Quiz on     F1Fo ATP Synthase