Molecular Biochemistry I

Oxidative Phosphorylation: F1Fo ATPase


Textbook Reading: Voet & Voet, Chapter 22, especially p. 827-833.

Some recent articles (optional reading): 
R. A. Capaldi & R. Aggeler (2002) "Mechanism of the F1Fo-type of ATP synthase, a biological rotary motor," Trends in Biochem. Sci. 27: 154-160.
P. D. Boyer (2002) "A research journey with ATP Synthase," J. Biol. Chem. 277: 39045-39061.
J. Weber & A. E. Senior (2003) "ATP synthesis driven by proton transport in F1Fo-ATP synthase," FEBS Lett. 545: 61-70.
G. Oster & H. Wang (2003) "Rotary protein motors," Trends in Cell Biol. 13: 114-121.
R. H. Fillingame, C. M. Angevine & O. Y. Dmitriev (2003) "Mechanics of coupling proton movements to c-ring rotation in ATP synthase," FEBS Lett. 555: 29-34.
M. Karplus & Y. Q. Gao (2004) "Biomolecular motors: the F1-ATPase paradigm," Curr. Opinion in Struct. Biol. 14: 250-259.
K. Kinosita, K. Adachi & H. Itoh (2004) "Rotation of F1-ATPase: how an ATP-driven molecular machine may work," Annu. Rev. Biophys. Biomol. Struct. 33: 245-268.
C. Chen, Y. Ko, M. Delannoy, S. J. Ludtke, W. Chiu & P. L. Pedersen (2004) "Mitochondrial ATP Synthasome," J. Biol. Chem. 279: 31761-31768.
R. Kagawa, M. G. Montgomery, K. Braig, A. G. W. Leslie & J. E. Walker (2004) "The structure of bovine F1-ATPase inhibited by ADP and beryllium fluoride," EMBO J. 23: 2734-2744.
P. Falson, A. Goffeau, M. Boutry & J.-M. Jault (2004) "Structural insight into the cooperativity between catalytic and noncatalytic sites of F1-ATPase," Biochim. Biophys. Acta 1658: 133-140.
J. E. Walker & V. K. Dickson (2006) "The peripheral stalk of the mitochondrial ATP synthase," Biochim. Biophys. Acta 1757: 286-296.
J. Weber (2006) "ATP synthase: Subunit-subunit interactions in the stator stalk," Biochim. Biophys. Acta 1757: 1162-1170.
B. A. Feniouk, T. Suzuki & M. Yoshida (2006) "The role of subunit epsilon in the catalysis and regulation of FoF1-ATP synthase," Biochim. Biophys. Acta 1757: 326-338.

Potential Test Question:

Diagram and describe major features of the "Binding Change Mechanism" that has been proposed for the F1Fo ATPase.
How does the crystal structure of the F1 ATPase that you explored with Chime support this concept? What subunits of the E. coli F1Fo complex constitute the stator and what parts the rotor of this molecular motor?
Describe the experiment that confirmed the rotational mechanism.

Copyright 1998-2007 by Joyce J. Diwan. All rights reserved.

Lecture notes on
F1Fo ATP Synthase

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F1Fo ATP Synthase   

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