Homepage for Drug Discovery (CHEM-4330/6330)

and Drug Discovery Laboratory (CHEM-4340)

Instructor: Mark P. Wentland, Department of Chemistry and Chemical Biology

CHEM-4330 (undergraduate) and CHEM-6330 (graduate) will examine how bioinformatics, functional genomics and other modern biotechnologies are used to speed the discovery of new drugs, especially those small organic molecules to treat human diseases with large unmet therapeutic need. Special emphasis will be placed on molecular target identification and validation as well as high-throughput screening to identify a lead. Topics to be discussed will include transgenic mice, RNA interference, DNA and protein microarrays, homogenous time-resolved fluorescence bioassays, phage-display, combinatorial chemistry and parallel synthesis. 3 credit hours. Prerequisites - For CHEM-4330: CHEM-2220 or CHEM-2260 or permission of instructor; for CHEM-6330: CHEM-6210 or permission of instructor.

CHEM-4340 (Drug Discovery Laboratory associated with CHEM-4330) enables students to reduce to practice the chemical and biological aspects of high-throughput screening used to discover lead molecules. Colorimetric and fluorescence plate readers will be used in 96-well plate format to generate enzyme inhibition data for small libraries of organic molecules. Students will use these inhibition data and published X-ray structural data to develop a pharmacophore model and rationalize a structure-activity relationship. 1 credit hour. Prerequisite - CHEM-4330 or concurrent with CHEM-4330. Course topics in graphical form.

Example of a lecture module:  High Troughtput Screening (HTS)

Example of a laboratory experiment:  96-Well Plate Colorimetric Assay for Ki Determination of (±)-2-Benzyl­succinic Acid, an Inhibitor of Carboxypeptidase A

For enrolled students - detailed course information will be distributed via RPI LMS

Drug Discovery and DD Laboratory are required courses for Rensselaer's Bioinformatics and Molecular Biology Major and are electives for Chemistry, Biology, Biochemistry/Biophysics, and other majors.